Hybrid Methods

Adressing problems in biology is becoming more and more complex – as both the number of potentially interacting players in multi-subunit complexes but also the size of protein and their complexes rises. To gain information of these multi-subunit complexes the application of multiple techniques is a more promising approach than using isolated techniques alone.
As an example fitting high-resolution crystal structures of submodules into EM maps of holocomplexes can lead to near-atomic models of complex assemblies. Structural analysis of protein complexes in solution can be achieved by combining NMR data with small angle X-ray and/or neutron scattering experiments. Functional conformations of macromolecules and assemblies can be addressed by combining structural methods with single-molecule analysis, e.g. to probe conformational plasticity and dynamics from single molecule FRET data on the basis of a structural framework.
We therefore aim to integrate data from different methods to get a more complete picture of complex problems.